Entamoeba histolytica Project

E. histolytica Microsource screen

Protocol

Amoeba strain and growth conditions. Trophozoites of E. histolytica strain HM1:IMSS were axenically maintained in TYI-S-33 medium supplemented with 10% bovine serum and plated into 96-well microtiter plates at approximately 104 cells/well.
In vitro detection of antiamoebic activities.  Each test compound was added to 48 h-old cultures (in logarithmic phase) at a concentration of 1 micromolar.  DMSO- and metronidazole-treated cultures served as controls. Cultures were then incubated at 37°C for 60 h in GasPakTM EZ Anaerobe Gas Generating Pouch System (BD Biosciences, San Jose, CA). Trophozoite viability was determined after 60 h incubation using the CellTiter-Glo™ Luminescent Cell Viability Assay (Promega), according to the manufacturer's instructions.