S.mansoni data

The data below represents a phenotypic screen of 2,160 compounds (many of which are FDA-approved drugs) from the Microsource library. Screening was done at 1 μM against newly transformed schistosomula (NTS) of Schistosoma mansoni. Phenotypes were recorded at 7d for the primary screen, and at 24hr and 7d for the secondary screen. These compounds were selected to undergo in vitro testing against adult worms of S. mansoni.

Phenotypes:
Dead, Dark, Overactive, Rounded, Degenerating but mobile, Slow, and None

CommonName	Primary NTS screen 7d	Secondary NTS screen 24hr	Secondary NTS screen 7d
lasalocid sodium	Dead;Dead,Immobile	Dead	Dead;Degenerated but Mobile,Partial Death
gambogic acid	Dead	Dead	Dead
difractaic acid	Dead	Dead	Dead
Amitriptyline hydrochloride	Overactive	Overactive+	Overactive+
Sanguinarine chloride	Degenerated but Mobile,Rounded;Degenerated but Mobile	Overactive;Rounded	Dead;Rounded,Slow
Benztropine mesylate	Degenerated but Mobile	Rounded,Overactive
Cyclosporin A	Rounded	Overactive	Rounded,Overactive
salinomycin, sodium	Dead	Dead	Dead
triflupromazine hydrochloride	Overactive	Overactive
celastrol	Rounded	Overactive+	Dead
amphotericin b	Dead	Dead	Dead
Chlorpromazine hydrochloride	Degenerated but Mobile	Overactive++	Overactive
Clomipramine hydrochloride	Overactive	Overactive	Overactive
isorotenone	Immobile,Rounded	Rounded	Dead
dihydrogambogic acid	Degenerated but Mobile	Overactive+	Dead
tomatine	Partial Death	Dead	Dead
Niclosamide	Dead	Dead	Dead
dihydrorotenone	Rounded,Immobile	Overactive	Dark,Slow
digitonin	Partial Death	Dead	Dead
doxepin hydrochloride	Overactive	Overactive++	Overactive
fluorouracil	Slow		Degenerated but Mobile,Dark
Quinacrine dihydrochloride	Dark		Dark,Rounded
ethinyl estradiol	Overactive	Overactive	Overactive-
gramicidin	Dead-Mostly	Overactive-	Partial Death
promazine hydrochloride	Overactive	Overactive	Overactive
Imipramine hydrochloride	Overactive	Overactive+	Overactive
Neostigmine bromide	Slow	Rounded,Slow	Rounded,Dark
thiothixene	Dark,Slow	Rounded,Overactive
praziquantel	Degenerated but Mobile	Rounded,Overactive; Overactive,Small	Degenerated but Mobile (broken);Degenerated but Mobile
clofoctol	Dark	Rounded,Overactive	Rounded,Slow
anisomycin	Dead	Rounded,Overactive	Dead
cyclobenzaprine hydrochloride	Slightly Overactive	Overactive	Overactive
Taxol	Dark	Overactive-	Dark,Slow
nigericin sodium	Dead	Dead	Dead
hycanthone	Dead,Overactive	Dark,Overactive	Dead
methazolamide	Dark		Rounded,Dark
ribostamycin sulfate	Dark	Rounded
monensin sodium (monensin a is shown)	Dark	Dead	Dead
dibekacin	Dark		Dark,Slow,Dying
chlorprothixene hydrochloride	Overactive	Overactive++	Overactive-
triptonide	Dead	Rounded,Overactive	Dead
rhodomyrtoxin b	Dead	Dead	Dead
dimethyl gambogate	Dead		Dead
10-hydroxycamtothecin	Rounded		Rounded
dihydrocelastryl diacetate	Dead	Overactive	Dead
Fluoxetine hydrochloride	Overactive	Overactive++	Overactive-
Benzotript	Rounded,Dark,Slow		Dark
compactin	Dead		Dead
simvastatin	Dead		Dead
bithionol	Dead,Degenerated	Slow	Slow,Dark
thiram	Slow		Dark,Slow
2,3,29-triacetoxy-24-nor-1,3,5,7-friedelatetraene	Dead	Rounded,Overactive	Dead
cytochalasin q	Degenerated but Mobile	Dead	Dead
(-)-Physostigmine	Slow,Immobile	Rounded	Rounded
alexidine hydrochloride	Dead,Degenerated	Rounded	Rounded

The data below represents a phenotypic screen of 2,160 compounds (many of which are FDA-approved drugs) from the Microsource library. Screening was done at 1 µM against adult worms of Schistosoma mansoni. Phenotypes were recorded at 7hr, 24hr, 2d, 3d, 4d.

Phenotypes:
Dead, Laying on sides, Slow, Dark, Shrunken, Overactive, Sexes separate, Tegumental blebbing, and None

Common name	7 hr	24 hr	2 d	3 d	4 d
Alexidine hydrochloride	None	None	None	None	None
Amitriptyline hydrochloride	None	None	None	None	None
Amphotericin b	None	None	None	None	None
Anisomycin	None	Laying on sides	Laying on sides	Dark, Laying on sides	Dark, Laying on sides
Benztropine mesylate	None	None	None	None	None
Bithionol	None	Slow, Laying on sides	Slow, Laying on sides	Slow, Dark, Laying on sides	Slow, Dark, Laying on sides
Celastrol	None	Slow, Dark, Shrunken	Slow, Dark, Dead	Slow, Dark, Dead	Slow, Dark, Dead
Chlorpromazine hydrochloride	Overactive	Overactive	Overactive	Overactive	Overactive
Chlorprothixene hydrochloride	Overactive	None	None	None	None
Clofoctol	None	None	None	None	None
Clomiphene citrate	None	None	None	None	None
Clomipramine hydrochloride	Overactive	None	None	None	None
Cyclobenzaprine hydrochloride	Overactive	None	None	None	None
Cyclosporin A	Overactive	Overactive	Overactive	Slow, Laying on sides	Slow, Laying on sides
Cytochalasin q	None	None	None	None	None
Diffractaic acid	Slow, Dark, Laying on sides	Slow, Dark, Laying on sides	Slow, Dark, Laying on sides	Dead	Dead
Digitonin	None	Slow, Dark	Slow, Dark	Slow, Dark	Slow, Dark
Dihydrocelastryl diacetate	None	Sexes separate, Dark	Sexes separate, Dark	Slow, Dead	Slow, Dead
Dihydrogambogic acid	None	None	None	Slow, Laying on sides	Slow, Laying on sides
Dihydrorotenone	None	None	None	None	None
Doxepin hydrochloride	Overactive	Overactive	Overactive	Overactive	Overactive
Dyclonine hydrochloride	None	None	None	None	None
Epoxygedunin	None	None	None	None	None
Ergonovine maleate	None	None	None	None	None
Ethinyl estradiol	Overactive	Overactive	Overactive	Overactive	Dark, Slow
Fluoxetine hydrochloride	Overactive	None	None	None	None
Gambogic acid	Slow, Laying on sides	Slow, Dark, Laying on sides, Shrunken	Slow, Dark, Laying on sides, Shrunken	Slow, Dark, Laying on sides, Shrunken	Dead
Gramicidin	None	None	None	None	None
Hycanthone	None	None	Laying on sides, Slow	Laying on sides, Slow	Shrunken, Dead
Imipramine hydrochloride	Overactive	Overactive	Overactive	Overactive	Overactive
Isorotenone	None	None	None	None	None
Lasalocid sodium	Slow, Dark, Laying on sides	Slow, Dark, Laying on sides	Slow, Dark, Laying on sides	Slow, Dark, Laying on sides	Tegumental blebbing, Dead
Methomyl	None	None	None	None	None
Miconazole nitrate	None	None	None	None	None
Monensin sodium (monensin a is shown)	None	None	Slow, Laying on sides	Slow, Laying on sides	Slow, Laying on sides
Neostigmine bromide	None	None	None	None	None
Niclosamide	Slow, Shrunken	Slow, Shrunken	Tegumental blebbing, Dead	Tegumental blebbing, Dead	Tegumental blebbing, Dead
Nigericin sodium	None	Slow, Laying on sides	Slow, Laying on sides	Slow, Dark, Shrunken	Slow, Dark, Shrunken
Perseitol	None	None	None	None	None
(-)-Physostigmine	None	None	None	None	None
Praziquantel	Shrunken, Dark, Slow, Tegumental blebbing	Shrunken, Dark, Slow, Tegumental blebbing	Dead	Dead	Dead
Prazosin hydrochloride	None	None	None	None	None
Promazine hydrochloride	Overactive	Overactive	Overactive	Overactive	Overactive
Pyrromycin	None	None	None	None	None
Pyrvinium pamoate	None	Tegumental blebbing	Tegumental blebbing	Tegumental blebbing	Dead
Rhodomyrtoxin b	Shrunken, Dark, Slow	Tegumental blebbing, Dead	Tegumental blebbing, Dead	Tegumental blebbing, Dead	Tegumental blebbing, Dead
Ribostamycin sulfate	None	None	None	None	None
Salinomycin, sodium	None	Slow, Laying on sides	Slow, Laying on sides	Slow, Laying on sides	Slow, Dark, Laying on sides
Sanguinarine chloride	None	None	None	None	None
Sisomicin sulfate	None	None	None	None	None
Taxol	None	None	None	None	None
Thiothixene	None	None	None	None	None
Tomatine	None	None	None	None	None
2,3,29-triacetoxy-24-nor-1,3,5,7-friedelatetraene	Slow	Slow, Dark, Sexes separate	Slow, Dark, Sexes separate	Slow, Dark, Sexes separate	Dead
Triflupromazine hydrochloride	Overactive	None	None	None	None
Triptonide	None	Slow	Slow	Slow, Dark, Laying on sides	Slow, Dark, Laying on sides
(±)-Verapamil hydrochloride	None	None	None	None	None
Benzotript	None	None	None	None	None
Compactin	None	None	None	None	None
Dibekacin	None	None	None	None	None
Dimethyl gambogate	None	None	None	Slow, Dark	Slow, Dark
Fluorouracil	None	None	None	None	None
10-hydroxycamtothecin	None	None	None	None	None
Methazolamide	None	None	None	None	None
Quinacrine dihydrochloride	None	None	None	None	None
Simvastatin	None	None	None	None	None
Thiram	None	None	None	None	None